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–ÍÂÔÄ  .Â  qGACNNNNNNGTC,CCAGGCCTGG,GATCCGGATC,CCWGGTACCWGG,CCANNNNNTGG,CCAGGNNNTGG,CCANNNCCTGG,CCAGGNCCTGG,GCACNNNAACGTTNNNGTGC,CACNNNGTG,CCNNNNNNNGG,AACNAACRYGTGCGTGC,GCACGCACRYGTTNGTT,GCACNAACRYGTTNGTGC,AACGCACRYGTGCGTT,GACNNNNNGTC,GAANNNNNNNTTGG,CCAANNNNNNNTTC,CACNNNNGTG,AACACNNNNGTGC,GCACNNNNGTGTT,GCANNNNNNTGC,GAACNNNNNNTCC,GGANNNNNNGTTC,CAGNNNCTG,CCAGGNNCTG,CAGNNCCTGG,CCWGGGCCC,GGGCCCWGG,CCWGGGCCCWGG,AACNNNNNNGTGCACNNNNNNGTT,GACNNNGTC,GAANNNNTTC,CCWGGTACC,GGTACCWGG,CCWGGWCCWGG,ACNNNNGTAYC,GRTACNNNNGT,CCWGGYRCCWGG,GAAGNNNNNNTAC,GTANNNNNNCTTC,AACGGCNNNGTGC,GCACNNNGCCGTT,GCACGGCNNNGTT,AACNNNGCCGTGC,CGANNNNNNTGC,GCANNNNNNTCG,CGATCNNNNTGC,GCANNNNGATCG,TGANNNNNNTCA,TGATCNNNNTCA,TGANNNNGATCA,TGATCNNGATCA,AACCTGCNNGTGC,GCACNNGCAGGTT,GCACCTGCNNGTT,AACNNGCAGGTGC,GCCNNNNNGGC,AACNNNNNNGTGCMC,GKGCACNNNNNNGTT,CCWGGNCCWGG,AACTGGGNNGTGC,GCACNNCCCAGTT,GCACTGGGNNGTT,AACNNCCCAGTGC,GACNNNNGTC,GAGNNNNNCTC,CCWGGTCTC,GAGACCWGG,GATNNNNATC,GATCNNNATC,GATNNNGATC,GATCNNGATC,CCWGGCGCCWGG,ACNNNNNCTCC,GGAGNNNNNGT,AACNNNNNNGTGCAG,CTGCACNNNNNNGTT,GATCATGATC,CCWGGNNCCWGG,AACTGGNNNGTGC,GCACNNNCCAGTT,GCACTGGNNNGTT,AACNNNCCAGTGC,AACNAACCGGTGCGTGC,GCACGCACCGGTTNGTT,GCACNAACCGGTTNGTGC,AACGCACCGGTGCGTT,GCANNNNNTGC,GCACNNNNNNGTTCGAACNNNNNNGTGC,CCTNNNNNAGG,GCNNNNNNNGC,CCANNNNNNTGG,CCAGGNNCCTGG,CCTGGCCAGG,CAANNNNNGTGG,CCACNNNNNTTG,TTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAA,GCACNNNNNNGTTTAAACNNNNNNGTGC,CCAGGNCCY,RGGNCCTGG,CCWGGGNCCTGG,CCAGGNCCCWGG,AAGNNNNNCTT,GAYNNNNNVTC,GABNNNNNRTC,GAYNNNNNRTC,GATCNNNNVTC,GABNNNNGATC,GAYNNNNGATC,GATCNNNNRTC,GATCNNNGATC,GTYAACNNNNNNGTGC,GCACNNNNNNGTTRAC,GCACNNNNNNGTTAACNNNNNNGTGC,GATCNGATC,TAACTATAACGGTCCTAAGGTAGCGA,TCGCTACCTTAGGACCGTTATAGTTA,CTCTCTTAAGGTAGC,GCTACCTTAAGAGAG,TAGGGATAACAGGGTAAT,ATTACCCTGTTATCCCTA,GTTAACNNNNNNGTGC,GCACNNNNNNGTTAAC,AACNAACGCGTGCGTGC,GCACGCACGCGTTNGTT,GCACNAACGCGTTNGTGC,AACGCACGCGTGCGTT,TCCAACNNNNNNGTGC,GCACNNNNNNGTTGGA,CAYNNNNRTG,AACAYNNNNGTGC,GCACNNNNRTGTT,GTTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAAC,CCWGGCGCC,GGCGCCWGG,CCWGGNNCC,GGNNCCWGG,GATCGCGATC,AACNAACATGTGCGTGC,GCACGCACATGTTNGTT,GCACNAACATGTTNGTGC,AACGCACATGTGCGTT,TGGCAAACAGCTATTATGGGTATTATGGGT,ACCCATAATACCCATAATAGCTGTTTGCCA,ATCTATGTCGGGTGCGGAGAAAGAGGTAATGAAATGG,CCATTTCATTACCTCTTTCTCCGCACCCGACATAGAT,AACNNGCACGTGCNNGTT,GAACNNNNNCTC,GAGNNNNNGTTC,CCAGGWCCY,RGGWCCTGG,CCWGGGWCCTGG,CCAGGWCCCWGG,CCAGGWCCTGG,TTATAACNNNNNNGTGC,GCACNNNNNNGTTATAA,GCACNNNNNNGTTATAACNNNNNNGTGC,GAACNNNNNNTAC,GTANNNNNNGTTC,GGCCNNNNNGGCC,AACNNACTAGTGC,GCACTAGTNNGTT,AACTAGTNNGTGC,GCACNNACTAGTT,AACNAACTAGTGCGTGC,GCACGCACTAGTTNGTT,GCACNAACTAGTTNGTGC,AACGCACTAGTGCGTT,AACNNNAACGTGCNNGTGC,GCACNNGCACGTTNNNGTT,GCACCCANNNGTT,AACNNNTGGGTGC,TTAACNNNNNNGTGC,GCACNNNNNNGTTAA,CACNNNNNNTCC,GGANNNNNNGTG,CACNNNNGATCC,GGATCNNNNGTG,GATCTAGATC,CCANNNNNNNNNTGG,CCAGGCCT,AGGCCTGG,CCTCGAGG,GATCCGGA,TCCGGATC,GGCGCGCC,GCGATCGC,CCWGGWCC,GGWCCWGG,GATCGATC,GATCATGA,TCATGATC,GCGGCCGC,CCTGGCCR,YGGCCAGG,CCWGGNCC,GGNCCWGG,GGCCGGCC,RTGCGCAY,GATCNNGA,TCNNGATC,CGCGCGCG,CCTGGCCA,TGGCCAGG,CGCCGGCG,GTTTAAAC,GATCGCGA,TCGCGATC,TTAATTAA,VCTCGAGB,CCTGCAGG,CRCCGGYG,CGTCGACG,ATTTAAAT,GCCCGGGC,GATCTAGA,TCTAGATC,CACCTGC,GCAGGTG,CCTNAGG,GATCGAT,ATCGATC,CCTCAGC,GCTGAGG,GCTNAGC,CCTNAGC,GCTNAGG,GCTCTTC,GAAGAGC,GGTNACC,CGGWCCG,ACCWGGT,RGGNCCY,CCTGGAG,CTCCAGG,GGTGATC,GATCACC,GATCNGA,TCNGATC,GGGWCCC,GAAGATC,GATCTTC,CCCWGGG,TCCNGGA,TCCWGGA,RGGWCCY,TTATAA,AGGCCT,GACGTC,GTMKAC,TCCGGA,TGCGCA,ACCTGC,GCAGGT,GGTACC,AGTACT,GGYRCC,CCGCTC,GAGCGG,AACGTT,YGGCCR,RAATTY,CTGAAG,CTTCAG,CACGTG,GRCGYC,AGCGCT,CTTAAG,ACRYGT,ACCGGT,ACTAGT,CACGTC,GACGTG,GWGCWC,GTGCAC,CYCGRG,GGGCCC,ATTAAT,CCTAGG,TTCGAA,GCTAGC,GKGCMC,TGGCCA,GGATCC,GRGCYC,ATCGAT,CACGAG,CTCGTG,GGCGCC,GAAGAC,GTCTTC,GCATGC,GTATCC,GGATAC,TGATCA,ACTGGG,CCCAGT,CTRYAG,RGCGCY,AGATCT,GGNNCC,CTGGAG,CTCCAG,CTTGAG,CTCAAG,CGATCG,GGTCTC,GAGACC,YACGTR,CCNNGG,GAATGC,GCATTC,WCCGGW,GCAATG,CATTGC,RCCGGY,GCGCGC,GAGGAG,CTCCTC,CGGCCG,CCCAGC,GCTGGG,GTGCAG,CTGCAC,CGRYCG,CGTACG,CGTCTC,GAGACG,CCATGG,TCGCGA,GDGCHC,TGTACA,TCATGA,ACATGT,GTATAC,CCWWGG,CTCTTC,GAAGAG,GCNNGC,CCRYGG,GTTAAC,RCATGY,TACGTA,RGATCY,GCGATG,CATCGC,GCAGTG,CACTGC,CCCGGG,CCGCGG,TTTAAA,GGCGGA,TCCGCC,GAGCTC,GATATC,CTGRAG,CTYCAG,CAGCAG,CTGCTG,GAATTC,ATGCAT,CATATG,GTYRAC,AAGCTT,GTNNAC,TCNNGA,CAATTG,ACGCGT,TCCRAC,GTYGGA,GCCGGC,CMGCKG,GCCGAG,CTCGGC,CTCGAG,CTGCAG,CAGCTG,GTCGAC,CTYRAG,AATATT,GATCGA,TCGATC,GACCGA,CACCCA,TCGGTC,TGGGTG,WGTACW,TARCCA,TGGYTA,TCTAGA,GGATC,GATCC,CCWGG,GTCTC,GAGAC,GCWGC,GGNCC,CCSGG,GGTGA,TCACC,GGWCC,GCAGC,GCTGC,CCATC,GATGG,ACGGC,GCCGT,GCNGC,CCNGG,GCATC,GATGC,ACTGG,CCAGT,GGATG,CATCC,CTCAG,CTGAG,GGGAC,GTCCC,ACNGT,CTNAG,CAGTG,CACTG,GACGC,GCGTC,CCCGC,GCGGG,GANTC,CGWCG,TCNGA,CCTTC,GAAGG,GTNAC,GAAGA,TCTTC,GAGTC,GACTC,GTSAC,GAWTC,GCSGC,CASTG,ATGAA,TTCAT,ACGGA,TCCGT,CGCG,CCGC,GCGG,GTAC,AGCT,GCGC,CTAG,GATC,GGCC,CCGG,CATG,RGCY,YATR,ACGT,TGCA,AATT,CCTC,GAGG,TTAA,ASST,TCGA                                                                                                                                                                                                                                                                                                                                                       "                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
                                                                                                                                  	            
                                                                                                                                                                          
               	   
         0                                                      	                                                	               #          )   *                  h   z      '   &         $   l                                                                                                                                                                  "                                                                                                                                                                                                                                                                                                                                                   
                                              	    
                                                            
     	 
   0                  	                	     #    ) *      h z  ' &   $ l   ~m  s  …  Ñ  „  ‰  �Ÿ�&¸C4ö8úû
# Ã4Óö@	
båËâˆš2ýE,¹!Aã	Ø§7Ût#¼Ñ,n0(_ W
»—  Z	,7@ù	ÓG s Ö 
.Á7@wž§ƒù	è	
&
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*�0t}‚6±»?{í0CüTb2ãbè2b2bb÷0—6õ·‘«-öY	ý�ÈŠ@	zz@	”ê¬2ô¥{)Ov„BÏF	I	æ$Ë�÷¹Äé×ŠË$ÂøºêøºO„Fæ{RÈŠÍ×;	B;âH
	 
|yV—P	ITr P	Ô
fk
ëô¨ –ÜQ À{|	k®cœ’´¿kß%`'×éºùˆ	ËÚ Z–àór°ï-lYÌ¯f ôh—ìuh—uÙä›¦A	xwJ		 X ‡„ww]J		«h—uÀ$ wô¶J	²
¬	ë­ˆš³8ú)û¶ü¾tçÞ9û”	¹T_	
Þ•Aç¦^
f
–àór°ï-l»-r $ÌWvwõ…_·G!	I	J	P	³
¿
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«Ö	æ¾ßfj‘ý 1ÌŽÝ÷¹žû¬Æ*E?ì	Ãjï›ãˆ×Zû
w ÂÃd½ðˆåc¨J§%	ž	ð²’âNá‚FÞJü”
âÛ r	•{„¾‹> 	¦,{ì	.Ã^æ½c%	² w]ô¶J	²
¬	«ôh—ìu+AHïßÌ¯×B;½c%	²ÃG|Þ	zí)& C Û –Eá	r	Ì	Ð	ä	[
i
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iˆî‘‚ÄK{ öd½ðc¨%	ð²þFÌô¯×$ŠëÙ°ÁÁÍ#.ûMG �©d¨›ó§º�™Õg¤ Åý 1`'Ì×éŽºùˆÝ÷	¹ËžûÚ £¼F’H~ü×þ;	ÿ‡×þr;	ø
/@B™ÿ‡;ÙhimÊ—�u6 í«âuª7Al�	ÞÁ*HÉ¥.yïÜß)|Þ@ÆG�;Ç)9ñ”
$ d¹“*kx¨Uì-	Î	©¹f[·A	•$ ¹“*UìÎ	;	¬A	Æ*?EË0ê¬ÌMii-;ý”+IˆrT™>
™É$é«°ñÁ#y%‹ìÚbÂÎ/x ÃùË»�Ÿ	•¾kTçðªQ›Üžã†)2ó# »’w­ÞTº	
G
Meñ�·ÓP’ HZýPXwxR¥…CgÇÐGÆ	)	J	K	ã		
ç%Ë©º	‰ÄKM	%Èë}5Qx~q,îñ
wåÈ`&è›hYP¥…gÇGÆ)	çË©# ’w­ÞTº	
G
Me�·ÓP Zý»ñ’HIg�#)_å…N¤O¶xô
t“Ò
~	3Ê# Ï»’mwz}§ª­ÞT/	º	
#
1
G
më Me„ñ�·ÓPN3’­ HZé„úýx Ãù‰Ë»ÄK�M	Ÿ	%Èë}5•÷pªÌŸj�î°â�›_}–„<X; 0«9ûz	”	Q¹­d�›	 ý\Òüµ¾w\‚š|s’GàR¢	À	«íÍü†è3õo×TB^�¹{æ9†Hgû	Yòü‹W¨äm:}€¹�Öè3õ¦“ìoZ[×TB^�H nnŽ/8úy	©m/ñ
®þ
J
 cå	j
Bž¨Î´C f4
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  é<?xml version="1.0"?><Features nextValidID="16"><Feature recentID="0" name="AmpR promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5820-5924" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="AmpR" directionality="1" translationMW="31558.16" type="CDS" allowSegmentOverlaps="0" cleavageArrows="5993" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment name="signal sequence" range="5925-5993" color="#ccffcc" type="standard" translated="1"/><Segment range="5994-6785" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"/></Q></Feature><Feature recentID="2" name="lac promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-35" range="127-132" color="#ffffff" type="standard"/><Segment range="133-150" color="#ffffff" type="standard"/><Segment name="-10" range="151-157" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="CAP binding site" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="91-112" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="lac operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="165-181" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6956-7544" color="#ffff00" type="standard"/><Q name="direction"><V text="RIGHT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="f1 ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2584-3039" color="#ffff00" type="standard"/><Q name="direction"><V text="RIGHT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="T7 promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2399-2417" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="T3 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="226-244" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T3 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="URA3 promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3974-4189" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;S. cerevisiae URA3&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="URA3" directionality="2" translationMW="29256.26" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="3170-3973" color="#ff7f50" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;S. cerevisiae URA3&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;yeast auxotrophic marker, counterselectable with 5-fluoroorotic acid (5-FOA)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;orotidine-5'-phosphate decarboxylase, required for uracil biosynthesis&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELLELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQYSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGEYTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDDVVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN*"/></Q></Feature><Feature recentID="11" name="2Î¼ ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4451-5793" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;yeast 2Î¼ plasmid origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="M13 fwd" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2427-2443" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="M13 rev" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="189-205" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="SK primer" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="281-297" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="FRT" directionality="1" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4783-4830" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;FLP recombinase from the &lt;i&gt;Saccharomyces cerevisiae&lt;/i&gt; 2Î¼ plasmid&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;FLP-mediated recombination occurs in the 8-bp core sequence TCTAGAAA (&lt;a href=&quot;http://www.ncbi.nlm.nih.gov/pubmed/21891781&quot; title=&quot;http://www.ncbi.nlm.nih.gov/pubmed/21891781&quot;&gt;Turan and Bode, 2011&lt;/a&gt;).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
  #Ï<?xml version="1.0"?><Primers nextValidID="17"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="Amp-R" sequence="ATAATACCGCGCCACATAGC" description="Ampicillin resistance gene, reverse primer"><BindingSite location="6142-6161" boundStrand="1" annealedBases="ATAATACCGCGCCACATAGC" meltingTemperature="58"><Component hybridizedRange="6142-6161" bases="ATAATACCGCGCCACATAGC"/></BindingSite><BindingSite simplified="1" location="6142-6161" boundStrand="1" annealedBases="ATAATACCGCGCCACATAGC" meltingTemperature="58"><Component hybridizedRange="6142-6161" bases="ATAATACCGCGCCACATAGC"/></BindingSite></Primer><Primer recentID="1" name="F1ori-F" sequence="GTGGACTCTTGTTCCAAACTGG" description="F1 origin, forward primer"><BindingSite location="2880-2901" boundStrand="0" annealedBases="GTGGACTCTTGTTCCAAACTGG" meltingTemperature="57"><Component hybridizedRange="2880-2901" bases="GTGGACTCTTGTTCCAAACTGG"/></BindingSite><BindingSite simplified="1" location="2880-2901" boundStrand="0" annealedBases="GTGGACTCTTGTTCCAAACTGG" meltingTemperature="57"><Component hybridizedRange="2880-2901" bases="GTGGACTCTTGTTCCAAACTGG"/></BindingSite></Primer><Primer recentID="2" name="F1ori-R" sequence="AGGGAAGAAAGCGAAAGGAG" description="F1 origin, reverse primer"><BindingSite location="2670-2689" boundStrand="1" annealedBases="AGGGAAGAAAGCGAAAGGAG" meltingTemperature="57"><Component hybridizedRange="2670-2689" bases="AGGGAAGAAAGCGAAAGGAG"/></BindingSite><BindingSite simplified="1" location="2670-2689" boundStrand="1" annealedBases="AGGGAAGAAAGCGAAAGGAG" meltingTemperature="57"><Component hybridizedRange="2670-2689" bases="AGGGAAGAAAGCGAAAGGAG"/></BindingSite></Primer><Primer recentID="3" name="GFP-F" sequence="GGTCCTTCTTGAGTTTGTAAC" description="GFP, forward primer. Does NOT anneal to EGFP"><BindingSite location="1445-1465" boundStrand="0" annealedBases="GGTCCTTCTTGAGTTTGTAAC" meltingTemperature="54"><Component hybridizedRange="1445-1465" bases="GGTCCTTCTTGAGTTTGTAAC"/></BindingSite><BindingSite simplified="1" location="1445-1465" boundStrand="0" annealedBases="GGTCCTTCTTGAGTTTGTAAC" meltingTemperature="54"><Component hybridizedRange="1445-1465" bases="GGTCCTTCTTGAGTTTGTAAC"/></BindingSite></Primer><Primer recentID="4" name="GFP-R" sequence="CCATCTAATTCAACAAGAATTGGGACAAC" description="GFP, reverse primer. Does NOT anneal to EGFP"><BindingSite location="822-850" boundStrand="1" annealedBases="CCATCTAATTCAACAAGAATTGGGACAAC" meltingTemperature="58"><Component hybridizedRange="822-850" bases="CCATCTAATTCAACAAGAATTGGGACAAC"/></BindingSite><BindingSite simplified="1" location="822-850" boundStrand="1" annealedBases="CCATCTAATTCAACAAGAATTGGGACAAC" meltingTemperature="58"><Component hybridizedRange="822-850" bases="CCATCTAATTCAACAAGAATTGGGACAAC"/></BindingSite></Primer><Primer recentID="5" name="L4440" sequence="AGCGAGTCAGTGAGCGAG" description="L4440 vector, forward primer"><BindingSite location="7697-7714" boundStrand="0" annealedBases="AGCGAGTCAGTGAGCGAG" meltingTemperature="58"><Component hybridizedRange="7697-7714" bases="AGCGAGTCAGTGAGCGAG"/></BindingSite><BindingSite simplified="1" location="7697-7714" boundStrand="0" annealedBases="AGCGAGTCAGTGAGCGAG" meltingTemperature="58"><Component hybridizedRange="7697-7714" bases="AGCGAGTCAGTGAGCGAG"/></BindingSite></Primer><Primer recentID="6" name="M13 Forward" sequence="TGTAAAACGACGGCCAGT" description="In lacZ gene. Also called M13-F20 or M13 (-21) Forward"><BindingSite location="2426-2443" boundStrand="1" annealedBases="TGTAAAACGACGGCCAGT" meltingTemperature="57"><Component hybridizedRange="2426-2443" bases="TGTAAAACGACGGCCAGT"/></BindingSite><BindingSite simplified="1" location="2426-2443" boundStrand="1" annealedBases="TGTAAAACGACGGCCAGT" meltingTemperature="57"><Component hybridizedRange="2426-2443" bases="TGTAAAACGACGGCCAGT"/></BindingSite></Primer><Primer recentID="7" name="M13 Reverse" sequence="CAGGAAACAGCTATGAC" description="In lacZ gene. Also called M13-rev"><BindingSite location="188-204" boundStrand="0" annealedBases="CAGGAAACAGCTATGAC" meltingTemperature="49"><Component hybridizedRange="188-204" bases="CAGGAAACAGCTATGAC"/></BindingSite><BindingSite simplified="1" location="188-204" boundStrand="0" annealedBases="CAGGAAACAGCTATGAC" meltingTemperature="49"><Component hybridizedRange="188-204" bases="CAGGAAACAGCTATGAC"/></BindingSite></Primer><Primer recentID="8" name="M13/pUC Forward" sequence="CCCAGTCACGACGTTGTAAAACG" description="In lacZ gene"><BindingSite location="2435-2457" boundStrand="1" annealedBases="CCCAGTCACGACGTTGTAAAACG" meltingTemperature="61"><Component hybridizedRange="2435-2457" bases="CCCAGTCACGACGTTGTAAAACG"/></BindingSite><BindingSite simplified="1" location="2435-2457" boundStrand="1" annealedBases="CCCAGTCACGACGTTGTAAAACG" meltingTemperature="61"><Component hybridizedRange="2435-2457" bases="CCCAGTCACGACGTTGTAAAACG"/></BindingSite></Primer><Primer recentID="9" name="M13/pUC Reverse" sequence="AGCGGATAACAATTTCACACAGG" description="In lacZ gene"><BindingSite location="169-191" boundStrand="0" annealedBases="AGCGGATAACAATTTCACACAGG" meltingTemperature="58"><Component hybridizedRange="169-191" bases="AGCGGATAACAATTTCACACAGG"/></BindingSite><BindingSite simplified="1" location="169-191" boundStrand="0" annealedBases="AGCGGATAACAATTTCACACAGG" meltingTemperature="58"><Component hybridizedRange="169-191" bases="AGCGGATAACAATTTCACACAGG"/></BindingSite></Primer><Primer recentID="10" name="pBluescriptSK" sequence="TCTAGAACTAGTGGATC" description="For pBluescript vector"><BindingSite location="280-296" boundStrand="0" annealedBases="TCTAGAACTAGTGGATC" meltingTemperature="45"><Component hybridizedRange="280-296" bases="TCTAGAACTAGTGGATC"/></BindingSite><BindingSite simplified="1" location="280-296" boundStrand="0" annealedBases="TCTAGAACTAGTGGATC" meltingTemperature="45"><Component hybridizedRange="280-296" bases="TCTAGAACTAGTGGATC"/></BindingSite></Primer><Primer recentID="11" name="pBR322ori-F" sequence="GGGAAACGCCTGGTATCTTT" description="pBR322 origin, forward primer"><BindingSite location="7444-7463" boundStrand="0" annealedBases="GGGAAACGCCTGGTATCTTT" meltingTemperature="56"><Component hybridizedRange="7444-7463" bases="GGGAAACGCCTGGTATCTTT"/></BindingSite><BindingSite simplified="1" location="7444-7463" boundStrand="0" annealedBases="GGGAAACGCCTGGTATCTTT" meltingTemperature="56"><Component hybridizedRange="7444-7463" bases="GGGAAACGCCTGGTATCTTT"/></BindingSite></Primer><Primer recentID="12" name="pBRforEco" sequence="AATAGGCGTATCACGAGGC" description="pBR322 vectors, upsteam of EcoRI site, forward primer"><BindingSite location="4391-4409" boundStrand="1" annealedBases="AATAGGCGTATCACGAGGC" meltingTemperature="57"><Component hybridizedRange="4391-4409" bases="AATAGGCGTATCACGAGGC"/></BindingSite><BindingSite simplified="1" location="4391-4409" boundStrand="1" annealedBases="AATAGGCGTATCACGAGGC" meltingTemperature="57"><Component hybridizedRange="4391-4409" bases="AATAGGCGTATCACGAGGC"/></BindingSite></Primer><Primer recentID="13" name="pGEX 3'" sequence="CCGGGAGCTGCATGTGTCAGAGG" description="pGEX vectors, reverse primer"><BindingSite location="4331-4353" boundStrand="0" annealedBases="CCGGGAGCTGCATGTGTCAGAGG" meltingTemperature="66"><Component hybridizedRange="4331-4353" bases="CCGGGAGCTGCATGTGTCAGAGG"/></BindingSite><BindingSite simplified="1" location="4331-4353" boundStrand="0" annealedBases="CCGGGAGCTGCATGTGTCAGAGG" meltingTemperature="66"><Component hybridizedRange="4331-4353" bases="CCGGGAGCTGCATGTGTCAGAGG"/></BindingSite></Primer><Primer recentID="14" name="pRS-marker" sequence="CGGCATCAGAGCAGATTGTA" description="pRS vectors, use to sequence yeast selectable marker"><BindingSite location="4212-4231" boundStrand="1" annealedBases="CGGCATCAGAGCAGATTGTA" meltingTemperature="54"><Component hybridizedRange="4212-4231" bases="CGGCATCAGAGCAGATTGTA"/></BindingSite><BindingSite simplified="1" location="4212-4231" boundStrand="1" annealedBases="CGGCATCAGAGCAGATTGTA" meltingTemperature="54"><Component hybridizedRange="4212-4231" bases="CGGCATCAGAGCAGATTGTA"/></BindingSite></Primer><Primer recentID="15" name="T3" sequence="GCAATTAACCCTCACTAAAGG" description="T3 promoter, forward primer"><BindingSite location="223-243" boundStrand="0" annealedBases="GCAATTAACCCTCACTAAAGG" meltingTemperature="53"><Component hybridizedRange="223-243" bases="GCAATTAACCCTCACTAAAGG"/></BindingSite><BindingSite simplified="1" location="223-243" boundStrand="0" annealedBases="GCAATTAACCCTCACTAAAGG" meltingTemperature="53"><Component hybridizedRange="223-243" bases="GCAATTAACCCTCACTAAAGG"/></BindingSite></Primer><Primer recentID="16" name="T7" sequence="TAATACGACTCACTATAGGG" description="T7 promoter, forward primer"><BindingSite location="2397-2416" boundStrand="1" annealedBases="TAATACGACTCACTATAGGG" meltingTemperature="50"><Component hybridizedRange="2397-2416" bases="TAATACGACTCACTATAGGG"/></BindingSite><BindingSite simplified="1" location="2397-2416" boundStrand="1" annealedBases="TAATACGACTCACTATAGGG" meltingTemperature="50"><Component hybridizedRange="2397-2416" bases="TAATACGACTCACTATAGGG"/></BindingSite></Primer></Primers>
  <Notes>
<UUID>bb0299e9-ffa3-4dfb-b98e-1a848b4c0cf0</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>pRS426GFP-2Ã—PH(PLCÎ´)</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Created UTC="0:34:25">2021.5.13</Created>
<LastModified UTC="0:34:25">2021.5.13</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
<References>
<Reference date="2002-02" title="The yeast synaptojanin-like proteins control the cellular distribution of phosphatidylinositol (4,5)-bisphosphate." journal="Mol Biol Cell. 2002 Feb;13(2):542-57." pubMedID="11854411" type="Journal Article" journalName="Mol Biol Cell." authors="Stefan CJ, Audhya A, Emr SD" pages="542-57" volume="13(2)"/>
</References>
</Notes>
  Y     –         
?????                                                                                                       ÿ d      HO  d               E              ÿÿÿÿYô? P                                                                                                                                                              3<?xml version="1.0"?><EnzymeVisibilities vals=""/>
   )<?xml version="1.0"?><CustomEnzymeSets/>
